1/16/2024 0 Comments Bio rad marker proteinThe following replacement is recommended: Precision Plus Protein Unstained Protein Standards, Strep-tagged recombinant, 1 ml ( 1610363 ) SDS. Please contact technical support for more information on replacement product. Mix thoroughly, to ensure that the solution is homogeneous. 200 l, broad range standards (6,500200,000 MW), unstained mixture of nine proteins.Thaw the ladder either at room temperature or at 37-40☌ for a few minutes to dissolve precipitated solids.The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).ĥ00 µL Protein Marker sufficient for 100 mini gels or 50 large gels. In addition to providing visual verification of transfer at every step, stain-free imaging enables total protein normalization in each lane. Visualize total protein on gels and blots using Bio-Rad’s Stain-Free Gels featuring our proprietary Stain-Free Technology. Precision Plus Protein Dual Color standards contain ten protein bands of 10, 15, 20, 25, 37, 50, 75, 100, 150, and 250 kD. The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240 kDa. To verify protein transfer, stain the membrane with Ponceau S after blotting. Details BlueStar PLUS Prestained Protein Marker The supernatant was assayed for protein ( Bio - Rad ) and an aliquot containing 100 g of each specimen was precipitated with 6 volumes of -20 C acetone.
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